![Flow Cytometry: Human TLR4/NF-kB NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26276] - Surface staining of the TLR4 reporter cell line was analyzed by flow cytometry using a PE-conjugated anti-TLR4 antibody. Flow samples were prepared using the TLR Staining Flow Kit. Purple: Cells alone; Green: NF-kB/SEAPorterTM HEK 293 cell line stained with 1 ug anti-TLR4-PE; Red: TLR4 reporter cell line stained with 1 ug anti-TLR4-PE.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/10738_Human-TLR4-NF-kB-NF-kB-SEAP---(SEAPorter(TM))-Stable-Reporter-Cell-Line-Flow-Cytometry-NBP2-26276-img0005.jpg)
Flow Cytometry: Human TLR4/NF-kB NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26276] - Surface staining of the TLR4 reporter cell line was analyzed by flow cytometry using a PE-conjugated anti-TLR4 antibody. Flow samples were prepared using the TLR Staining Flow Kit. Purple: Cells alone; Green: NF-kB/SEAPorterTM HEK 293 cell line stained with 1 ug anti-TLR4-PE; Red: TLR4 reporter cell line stained with 1 ug anti-TLR4-PE.
![Ligand Activation: Human TLR4/NF-kB NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26276] - TLR4/NF-kB SEAPorterTM HEK 293 cells were plated in 24-well plates at 2 x 10^5 cells/well. After 16 h, cells were transiently transfected with control vector, MD2 plasmid or MD2/CD14 plasmid vector for 24 h. Cells were then stimulated with 0.5 ug/ml LPS for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: Both MD2 and CD14 are required for the complete LPS-mediated TLR4 activation.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/10739_Human-TLR4-NF-kB-NF-kB-SEAP---(SEAPorter(TM))-Stable-Reporter-Cell-Line-Ligand-Activation-NBP2-26276-img0006.jpg)
Ligand Activation: Human TLR4/NF-kB NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26276] - TLR4/NF-kB SEAPorterTM HEK 293 cells were plated in 24-well plates at 2 x 10^5 cells/well. After 16 h, cells were transiently transfected with control vector, MD2 plasmid or MD2/CD14 plasmid vector for 24 h. Cells were then stimulated with 0.5 ug/ml LPS for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: Both MD2 and CD14 are required for the complete LPS-mediated TLR4 activation.